http://www.bjvm.org/index.php/home/issue/feed 2025-07-31T10:37:17+00:00 Prof. A. K. M. Anisur Rahman arahman_med@bau.edu.bd Open Journal Systems Bangladesh Journal of Veterinary Medicine (BJVM) http://www.bjvm.org/index.php/home/article/view/159 2025-07-31T10:37:17+00:00 Rajesh Gautam et.rajesh01@gmail.com Bhaju Ram Mahato bhajumahato@gmail.com Amrit Shrestha cresthaamrit08@gmail.com Suman Kumar Singh suman.singh@pakc.tu.edu.np Muhammad Adil madilnawaz143@gmail.com Syed Mubash Sher Tajmir syedmubasher658@gmail.com Md. Mahfuzul Haque mahfuzul.dst@sau.ac.bd

<p style="font-weight: 400;"><strong>Background</strong>: The heating rate during the thawing of frozen semen significantly impacts the functional activation of mitochondria, which affects sperm motility assessment.</p> <p style="font-weight: 400;"><strong>Methods</strong>: This study examined the effect of thawing temperature on the motility of spermatozoa in cryopreserved semen. A total of 240 semen straws (0.25 ml each) from 24 bulls (8 Jersey, 8 Holstein Friesian (HF), and 8 Murrah breed), aged 2 to 6 years, were used. Semen was collected, analyzed, processed, frozen, and stored in liquid nitrogen using a standard protocol with a tris-citrate-egg yolk extender. Samples were thawed for 30 seconds in a water bath at temperatures T1: 32°C, T2: 34°C, T3: 36°C, T4: 38°C, and T5: 40°C. Sperm motility, progressive motility, fast motility, slow motility, and immotile percentage of the frozen semen were evaluated. A computer-assisted semen analyzer (CASA) was used for analysis. The data was entered into MS-Excel and analyzed using analysis of variance (ANOVA), with the significance between treatments assessed using Duncan’s multiple range test (DMRT) post hoc test.</p> <p style="font-weight: 400;"><strong>Results</strong>: Jersey semen showed the best motility percentage at T3 (p&lt;0.05) (T1: 53.025±6.73, T2: 60.01±3.81, T3: 74.33±1.40, T4: 65.59±3.17, T5: 59.92±3.58). HF semen also showed the best motility percentage at T3 (p&lt;0.05) (T1: 60.67±6.31, T2: 55.93±6.31, T3: 76.6±2.28, T4: 72.93±2.10, T5: 57.69±2.28). Murrah semen showed the best motility percentage at T4 (p&lt;0.05) (T1: 56.63±3.93, T2: 58.58±4.34, T3: 77.09±1.59, T4: 82.72±4.03, T5: 72.87±4.00). Progressive motility (%) was highest at T3 for Jersey (52.41±2.97), T3 for HF (56.29±4.65), and T4 for Murrah (63.94±6.37) (p&lt;0.05). Fast motility (%) was highest at T3 for Jersey (12.71±2.04), T3 for HF (18.43±1.63), and T4 for Murrah (12.589±2.74) (p&lt;0.05). Slow motility (%) was lowest at T3 for Jersey (27.8±3.43), T3 for HF (34.23±2.72), and T4 for Murrah (31.32±4.72) (p&lt;0.05). Immotility (%) was lowest at T3 for Jersey (25.73±1.37), T3 for HF (23.4±2.28), and T4 for Murrah (17.27±4.03) (p&lt;0.05).</p> <p style="font-weight: 400;"><strong>Conclusion:</strong> Thawing at T3 (36°C) enhances motility, progressive motility, and fast motility while reducing slow motility and immotile percentage in Jersey semen. Similarly, thawing at T4 (38°C) improves motility, progressive motility, and fast motility while reducing slow motility and immotile percentage in Murrah semen.</p>

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