https://www.bjvm.org/index.php/home Bangladesh Journal of Veterinary Medicine (BJVM) Bangladesh Society for Veterinary Medicine en-US Bangladesh Journal of Veterinary Medicine (BJVM) 1729-7893 https://www.bjvm.org/index.php/home/article/view/159 <p style="font-weight: 400;"><strong>Background</strong>: The heating rate during the thawing of frozen semen significantly impacts the functional activation of mitochondria, which affects sperm motility assessment.</p> <p style="font-weight: 400;"><strong>Methods</strong>: This study examined the effect of thawing temperature on the motility of spermatozoa in cryopreserved semen. A total of 240 semen straws (0.25 ml each) from 24 bulls (8 Jersey, 8 Holstein Friesian (HF), and 8 Murrah breed), aged 2 to 6 years, were used. Semen was collected, analyzed, processed, frozen, and stored in liquid nitrogen using a standard protocol with a tris-citrate-egg yolk extender. Samples were thawed for 30 seconds in a water bath at temperatures T1: 32°C, T2: 34°C, T3: 36°C, T4: 38°C, and T5: 40°C. Sperm motility, progressive motility, fast motility, slow motility, and immotile percentage of the frozen semen were evaluated. A computer-assisted semen analyzer (CASA) was used for analysis. The data was entered into MS-Excel and analyzed using analysis of variance (ANOVA), with the significance between treatments assessed using Duncan’s multiple range test (DMRT) post hoc test.</p> <p style="font-weight: 400;"><strong>Results</strong>: Jersey semen showed the best motility percentage at T3 (p&lt;0.05) (T1: 53.025±6.73, T2: 60.01±3.81, T3: 74.33±1.40, T4: 65.59±3.17, T5: 59.92±3.58). HF semen also showed the best motility percentage at T3 (p&lt;0.05) (T1: 60.67±6.31, T2: 55.93±6.31, T3: 76.6±2.28, T4: 72.93±2.10, T5: 57.69±2.28). Murrah semen showed the best motility percentage at T4 (p&lt;0.05) (T1: 56.63±3.93, T2: 58.58±4.34, T3: 77.09±1.59, T4: 82.72±4.03, T5: 72.87±4.00). Progressive motility (%) was highest at T3 for Jersey (52.41±2.97), T3 for HF (56.29±4.65), and T4 for Murrah (63.94±6.37) (p&lt;0.05). Fast motility (%) was highest at T3 for Jersey (12.71±2.04), T3 for HF (18.43±1.63), and T4 for Murrah (12.589±2.74) (p&lt;0.05). Slow motility (%) was lowest at T3 for Jersey (27.8±3.43), T3 for HF (34.23±2.72), and T4 for Murrah (31.32±4.72) (p&lt;0.05). Immotility (%) was lowest at T3 for Jersey (25.73±1.37), T3 for HF (23.4±2.28), and T4 for Murrah (17.27±4.03) (p&lt;0.05).</p> <p style="font-weight: 400;"><strong>Conclusion:</strong> Thawing at T3 (36°C) enhances motility, progressive motility, and fast motility while reducing slow motility and immotile percentage in Jersey semen. Similarly, thawing at T4 (38°C) improves motility, progressive motility, and fast motility while reducing slow motility and immotile percentage in Murrah semen.</p> Rajesh Gautam Bhaju Ram Mahato Amrit Shrestha Suman Kumar Singh Muhammad Adil Syed Mubash Sher Tajmir Md. Mahfuzul Haque Copyright (c) 2025 Bangladesh Society for Veterinary Medicine http://creativecommons.org/licenses/by/4.0/ 2025-07-31 2025-07-31 23 1 1 6 https://www.bjvm.org/index.php/home/article/view/160 <p style="font-weight: 400;"><strong>Background:</strong> <em>Trypanosoma</em> spp. infection is a significant disease affecting both humans and animals, including cats. This study aimed to assess the prevalence of <em>Trypanosoma</em> spp. in domestic and stray cats in the Mymensingh district, Bangladesh.</p> <p style="font-weight: 400;"><strong>Methods:</strong> A total of 36 blood samples were collected from cats (16 males and 20 females), prepared as thin blood smears, and examined under a microscope at the Department of Parasitology, Bangladesh Agricultural University, Mymensingh, Bangladesh.</p> <p style="font-weight: 400;"><strong>Results:</strong> Out of the 36 samples, 3 (8.00%) were infected with <em>Trypanosoma</em> spp. Both young cats (&lt;1 year old) and adult cats (&gt;1 year) had an equal infection rate of 8.33% with Trypanosoma spp. The odds of infection were 1.66 times higher in females (10.0%) than in males (6.25%). Trypanosoma spp. was prevalent throughout the year, with rates of 7.6%, 8.33%, and 9.1% during the summer, rainy, and winter seasons, respectively. The odds of infection were 1.1 times higher in the rainy season compared to the summer, and 1.2 times higher in the winter season compared to the summer.</p> <p style="font-weight: 400;"><strong>Conclusion:</strong> This study suggests that <em>Trypanosoma</em> spp. infection in domestic and stray cats is a persistent issue regardless of age, sex, or season.</p> Sheikh Arafatur Rahman Noorjahan Begum A. K. M. Anisur Rahman Copyright (c) 2025 Bangladesh Society for Veterinary Medicine http://creativecommons.org/licenses/by/4.0/ 2025-08-06 2025-08-06 23 1 7 12